Licensing
Description
Basal bodies are supported by three fiber systems, a striated kinetodesmal fiber that arises from the proximal end of the basal body and curves upward and anteriorly within the longitudinal ridge to the cell's right side of a row of basal bodies. Extending posteriorly and also curving up into the longitudinal ridge is a ribbon of postciliary microtubules. The kd may extend past several more anterior basal bodies but the postciliary ribbon does not extend beyond the next posterior basal body or pair of basal bodies in the kinety. Only the more posterior of the two basal bodies within a single depression is associated with the ends of these two accessory structures. An indentation of the plasma membrane, the parasomal sac, lies to the cell's right of each basal body or pair of basal bodies. TEM taken on 1/5/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X. Bar = 0.2µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12068) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Sources
- NCBI Organism Classification
- Paramecium multimicronucleatum
- Cell Type
- cell by organism
- eukaryotic cell
- Eukaryotic Protist
- Ciliated Protist
- Cellular Component
- cell cortex
- microtubule basal body
- microfibril
- kinetodesmal fiber
- microtubule
Biological Context
- Biological Process
- cortical cytoskeleton organization
Attribution
- Name
- Richard Allen (University of Hawaii)
- Link
- http://www5.pbrc.hawaii.edu/allen/
Grouping
This image is part of a group.Imaging
- Image Type
- transmission electron microscopy (TEM)
- illumination by electrons
- Imaging Mode
- detection of electrons
- film
- Parameters Imaged
- electron density
- Source of Contrast
- stain with broad specificity
- Visualization Methods
- stain with broad specificity
- osmium tetroxide
- uranyl salt
- lead salt
- Processing History
- recorded image
- film
- Print from negative scanned for Photoshop.
- Data Qualifiers
- processed data
Sample Preparation
- Methods
- glutaraldehyde fixed tissue
- osmium tetroxide fixed tissue
- tissue in epoxy resin embedment
- microtome-sectioned tissue
- Relation To Intact Cell
- microtome-sectioned tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 2430px | —— |
| Y | 2202px | —— |