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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13093*  Cite 
Description

To characterize and validate the gluteraldehyde fixation method, a live amoeba syringe-loaded with Rhodamine-N58C-profilin-II was monitored in a flow cell as the fixative was introduced. This time series of cooled CCD fluorescence micrographs shows a cell as it is being fixed in 20% Dulbecco's PBS at pH 6.5 containing 1% glutaraldehyde at room temperature. See publication below for details. Live cells were observed with a Zeiss Axiovert 135 fluorescence microscope. Electronic imaging of fluorescent specimens was performed using a Photometrics cooled, charge-coupled device (CCD) camera (PXL-1400) controlled with IPLab Spectrum 3.1P software (Scanolytics, Fairfax, VA) on Macintosh computers. Copyright © 1999 by Company of Biologists and reproduced/adapted with permission in 2011.

Biological Sources
NCBI Organism Classification
Acanthamoeba castellanii
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
amoeboid cell
Cellular Component
lamellipodium
cytoplasm
endosome
Attribution
Names
Don Kaiser
Published
D. A. Kaiser, V. K. Vinson, D. B. Murphy & T.D. Pollard, J. Cell Sci. 112, 3779-3790 (1999)
Pubmed
10523513
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13093
Archival Resource Key (ARK)
ark:/b7295/w9cil13093
Grouping This image is part of a group.
Imaging
Image Type
absorption of illumination
Image Mode
UV/visible/IR photons
Parameters Imaged
UV/visible/IR illumination
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
fluorescent labels
syringe loaded with Rho-N58C-PII
Processing History
recorded image
intensified CCD (ICCD)
Data Qualifiers
processed data
Sample Preparation
Methods
unfixed tissue
Methods
living tissue
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 208px ——
Y 288px ——


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