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Description

A live amoeba treated with the membrane-binding vital dye FM4-64 which stains the plasma membrane, contractile vacuole and other organelles. Large membrane-bounded organelles are excluded from the broad, thin, lamellipodium as the cell extends a pseudopod toward the upper left. Live cells were observed with a Zeiss Axiovert 135 fluorescence microscope. Electronic imaging of fluorescent specimens was performed using a Photometrics cooled, charge-coupled device (CCD) camera (PXL-1400) controlled with IPLab Spectrum 3.1P software (Scanolytics, Fairfax, VA) on Macintosh computers. Copyright © 1999 by Company of Biologists and reproduced/adapted with permission in 2011.

Biological Sources

NCBI Organism Classification
Acanthamoeba castellanii
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
amoeboid cell
Cellular Component
contractile vacuole
plasma membrane
organelle membrane

Attribution

Name
Don Kaiser
Published
D. A. Kaiser, V. K. Vinson, D. B. Murphy & T.D. Pollard, J. Cell Sci. 112, 3779-3790 (1999)
Pubmed
10523513

Grouping

This image is part of a group.

Imaging

Image Type
absorption of illumination
Imaging Mode
UV/visible/IR photons
Parameters Imaged
UV/visible/IR illumination
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
fluorescent labels
membrane-binding vital dye FM4-64
Processing History
recorded image
intensified CCD (ICCD)
Data Qualifiers
processed data

Sample Preparation

Methods
unfixed tissue
Relation To Intact Cell
living tissue
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 144px ——
Y 144px ——
Time -- sec
*CIL – Cell Image Library accession number. Please use this to reference an image.