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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:1307*  Cite 
Description

High resolution image of immunogold labeled microtubules next to the cytoproct radiate from basal bodies and also from the tip of the cytoproct. These microtubules lie along the approaching spent DV. b-tubulin immunogold labeled TEM image taken on 7/5/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 12,000X. Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73. The raw film was scanned with a Nikon Coolscan 9000ED. This image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium tetraurelia
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cell cortex
cortical microtubule cytoskeleton
cortical microtubule
microtubule basal body
axonemal microtubule
Biological Context
Biological Process
microtubule cytoskeleton organization
Attribution
Names
Richard Allen (University of Hawaii)
Link
http://www5.pbrc.hawaii.edu/allen/
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL1307
Archival Resource Key (ARK)
ark:/b7295/w9cil1307
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
intrinsic mass distribution
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
gold
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
glutaraldehyde fixed tissue
tissue in vitreous ice embedment
microtome-sectioned tissue
monoclonal antibody
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 5599px 1.25nm
Y 3792px 1.25nm