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Description

Spiroplasmas are bacteria in the Class Mollicutes that are frequently associated with insects and/or plants. This confocal laser scanning micrographs show aggregates of symbiotic/ commensal spiroplasma-like organisms (SLOs) in the midgut (mg) and filter chamber (fc) of a leafhopper, Dalbulus maidis. Large accumulations of these SLOs (bright red areas, asterisks) were found in the gut lumen, but unlike the plant-pathogenic mollicutes, they did not seem to invade the gut epithelium or other tissues in their insect hosts. This image is related to Fig. 3 in Arch. Microbiol (2011) 193:35-44.

Biological Sources

NCBI Organism Classification
Bacteria: Spiroplasma, Class Mollicutes
Insect host: Dalbulus maidis (Hemiptera, Cicadellidae)
Cell Type
epithelial cell
bacteria
Cellular Component
nucleus
actin cytoskeleton

Biological Context

Biological Process
detection of bacterium

Attribution

Names
El-Desouky Ammar
Gail E. Gasparich
David G. Hall
Saskia A. Hogenhout
Published
Arch Microbiol (2011) 193:35塴
Pubmed
20978745

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
Imaging Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Alexa Fluor 633
propidium iodide
Processing History
unprocessed raw data

Sample Preparation

Methods
formaldehyde fixed tissue
permeabilized tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 787px 0.763μm
Y 1153px 0.763μm
Channel Wavelength
1 propidium iodide, Alexa 633 μm
*CIL – Cell Image Library accession number. Please use this to reference an image.