Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12629^* Cite

Description

Thick microtubule bundles are organized around the periphery of each stem cell in human embryonic stem cell colonies. Human embryonic fibroblasts were grown on a layer of mouse embryonic fibroblasts which provide nutrients and help maintain pluripotency of the colonies. The sample has been labeled with an antibody to tubulin using standard protocols for immunolabeling.

Technical Details

Images were deconvolved using Nikon Elements software.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: embryonic stem cell
Cellular Component: microtubule

Attribution

Names: Sabrina Lin
OTHER: UCR Stem Cell Center

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL12629
Archival Resource Key (ARK): ark:/b7295/w9cil12629

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: primary antibody plus labeled secondary antibody
Processing History: deconvolution

Sample Preparation

Methods: chemically fixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1600px	——
Y	1200px	——