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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12330*  Cite 
Description

Euplotes sp. (eurystomus ?) is a hypotrich. This ciliate has a few rows of dorsal cilia as well as specialized clumps of cilia called cirri on its ventral side and a complex of membranelles involved in feeding called the adoral zone of membranelles. The cell is flattened dorso-ventrally. This electron micrograph is a longitudinal section dividing dorsal from ventral parts of the cell and exposing the oral cavity, the thread-like macronucleus, the extended contractile vacuole, contractile vacuole pore, and a large assortment of food vacuoles many of which contained material that was lost during sectioning leaving holes in the section. The cytopharynx and nascent food vacuole along with the discoidal (pharyngeal) vesicles are also seen. TEM taken on 8/3/67 by R. Allen with Philips 200 operating at 60kV. Neg. 1,370X. Bar = 5µm. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution version of this image is in the library (CIL:38867) and is available for quantitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Euplotes sp.
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Euplotes sp. from Carolina Biological Supply
Cellular Component
cilium
cytoplasm
macronucleus
food vacuole
contractile vacuole
cytopharynx
Attribution
Names
Richard Allen
Link
http://www5.pbrc.hawaii.edu/allen/
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12330
Archival Resource Key (ARK)
ark:/b7295/w9cil12330
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 2674px ——
Y 3000px ——


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