Description

To determine the extent to which extracellular matrix proteins colocalize with the microtubule-anchoring factor LL5β, MCF-10A epithelial cells were immunostained with antibodies to the secreted autocrine laminin-5 (green), the laminin receptor integrin α6 (blue) and LL5β (red). Analyses suggest that LL5s are colocalized with activated pools of integrins associated with laminin-5. Overall findings from this publication showed that signaling from laminin-integrin associations plays a role in attaching microtubule plus ends to the epithelial basal cell cortex. This image is the original data file for Fig. 3 Panel D-b from J Cell Biol (2010) 189 (5):901-917.

Technical Details

MCF-10A epithelial cells were fixed with 1:1 mixture of methanol/acetone (-20° C, 10 min), permeabilized with 0.5% Triton in PBS, and immunostained sequentially with primary, then secondary antibodies. LL5β (Cy5, 633 nm laser), laminin-5 (rhodamine red-X, 543 nm laser)) and integrin α6 (Cy2, 488 nm laser, and observed using a confocal laser-scanning microscope system. For additional details see: J. Cell Biol. 2010. 189(5):901-917.