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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12286*  Cite 
Description

This confocal image of wild type cells plated on cell-derived matrices that correspond to an accompanying image of beta3-/- integrin cells. There are ncreased numbers of peripheral matrix-associated adhesions in the beta3-/- cells. The cells are co-stained for active beta1 integrin (9EG7-FITC, green), paxillin (Alexa-568, red) and fibronectin (Alexa-633, blue). Images were acquired using a Zeiss LSM 510 Meta microscope, 1024x1024 resolution, 13 stacks in the Z plane at 0.5um intervals. Images correspond to Fig 1C in J Cell Bio. 2010. 189:369-383

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
fibroblast
Cell Line
wild type corresponding to beta3 integrin -/-
Cellular Component
focal adhesion
extracellular matrix
cell derived 3D matrix
Biological Context
Biological Process
migration in 3D matrix
Attribution
Names
Daniel C. Worth
Kairbaan Hodivala-Dilke
Stephen D. Robinson
Samantha J. King
Penny E. Morton
Frank B. Gertler
Martin J. Humphries
Maddy Parsons
Published
J Cell Bio 2010. 189:369-383
Pubmed
20404115
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12286
Archival Resource Key (ARK)
ark:/b7295/w9cil12286
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Fluorescein (FITC)
Alexa Fluor 568
Alexa Fluor 633
primary antibody plus labeled secondary antibody
Processing History
color combine
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
chemically fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1024px 0.127µm
Y 1024px 0.127µm
Z —— 0.5µm
Channel Wavelength
1 488, 561, 633µm


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