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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12165*  Cite 
Description

Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase cell localization of exogenous TrAP-INCENP (red) plus endogenous Aurora B (green) and DNA (blue) in INCENPOFF cells expressing TrAP-INCENP WT. See Fig 6A panel 1 in Xu et al. 2009 J Cell Biol 187, 637-653.

Biological Sources
NCBI Organism Classification
Gallus gallus gallus
Cell Type
memory B cell
Cell Line
DT40
Cellular Component
nuclear chromosome
spindle
chromosome, centromeric region
Biological Context
Biological Process
mitosis
Attribution
Names
Z. Xu
H. Ogawa
P. Vagnarelli
J.H. Bergmann
D.F. Hudson
S. Ruchard
T. Fukagawa
W.C. Earnshaw
K. Samejima
Published
Xu et al. 2009 J Cell Biol 187, 637-653
Pubmed
19951914
Link
http://jcb-dataviewer.rupress.org/jcb/browse/...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12165
Archival Resource Key (ARK)
ark:/b7295/w9cil12165
Grouping This image is part of a group.
Imaging
Image Type
charge coupled device (CCD)
Image Mode
widefield illumination
Parameters Imaged
fluorescence emission
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
Fluorescein (FITC)
TexasRed
4',6-diamidino-2-phenylindole (DAPI)
Processing History
constrained iterative deconvolution
Data Qualifiers
processed data
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——
Z 26px ——


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