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Description

Cell of the DT40 chicken B lymphocyte line at metaphase. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. In inner centromere protein INCENP-OFF cells, levels of phosphorylated histone H3 (H3-S10ph, red, and H3-S28ph, green) are low compared to INCENP-ON cells. DAPI stained chromosomes shown in blue. See Fig 2A panel 13 in Xu et al. 2009 J Cell Biol 187, 637-653.

Biological Sources

NCBI Organism Classification
Gallus gallus gallus
Cell Type
memory B cell
Cell Line
DT40
Cellular Component
nuclear chromosome
spindle
chromosome, centromeric region

Biological Context

Biological Process
mitosis

Attribution

Names
Z. Xu
H. Ogawa
P. Vagnarelli
J.H. Bergmann
D.F. Hudson
S. Ruchard
T. Fukagawa
W.C. Earnshaw
K. Samejima
Published
Xu et al. 2009 J Cell Biol 187, 637-653
Link
http://jcb-dataviewer.rupress.org/jcb/...
Pubmed
19951914

Grouping

This image is part of a group.
Data Qualifiers
processed data

Sample Preparation

Methods
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——
Z 49 ——
*CIL – Cell Image Library accession number. Please use this to reference an image.