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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11963*  Cite 
Description

The movie illustrates two approaches to understanding the 3D structure of complex objects, using the pluteus stage embryo of the sea urchin Arbacia punctulata. The first sequence is a through-focus DIC series presented as a 'walk through' in the z dimension. The second uses projections at different angles through a z-stack, with the results presented as a 'rocking' series in which the 3D structure becomes more readily apparent.

Technical Details

See: K Okazaki and S Inoue (1976). Crystal properties of the larval sea urchin spicule. Dev Growth Diff 18:413-434; S Inoue and T Inoue (1986) Computer-aided stereomicroscopic video reconstruction and serial display from high-resolution light microscope optical sections. Ann NY Acad Sci 483:392-404.

Biological Sources
NCBI Organism Classification
Arbacia punctulata
Cell Type
embryonic cell
Biological Context
Biological Process
Development
Attribution
Names
Shinya Inoue
Published
S. Inoue 2008 Collected Works of Shinya Inoue. World Scientific Publishing Company, Singapore, Hackensack, NJ, USA
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL11963
Archival Resource Key (ARK)
ark:/b7295/w9cil11963
Imaging
Image Type
recorded image
Image Mode
differential interference contrast microscopy
Parameters Imaged
optical path length gradient
Source of Contrast
boundaries between regions with different refractive index
Visualization Methods
visualization of contiguous regions
Data Qualifiers
processed data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 720px ——
Y 480px ——