Licensing

Attribution_nc_sa
Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code
Comments: Powered by Pnyxe

Description

The time lapse movie spans fertilization, ooplasmic segregation, and early divisions in the egg of the tunicate Phalusia mammilata. Contrast is provided by both polarizing microscopy and differential interference microscopy. The entrance of the sperm (upper right) stimulates waves of contraction in the cytoplasm. Polar bodies are formed and extruded, after which male and female pronuclei migrate toward each other, and nuclear fusion occurs. Finally, the zygote proceeds through mitosis and cleavage stages.

Technical Details

See: Sardet et al. (1989) Fertilization and ooplasmic movements in the ascidian egg. Development 105:237-249.

Biological Sources

NCBI Organism Classification
Phallusia mammilata
(white sea-squirt)
Cell Type
egg
Cellular Component
spindle

Biological Context

Biological Process
fertilization
meiosis
mitosis

Attribution

Names
Shinya Inoue
Lionel Jaffe
Christian Sardet
Johanna Speksnijder
Published
S. Inoue 2008 Collected Works of Shinya Inoue. World Scientific Publishing Company, Singapore, Hackensack, NJ, USA
Data Qualifiers
processed data

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 720px ——
Y 480px ——
Time -- sec
*CIL – Cell Image Library accession number. Please use this to reference an image.