Licensing

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Description

The time lapse movie shows mitosis in a cell of the liquid endosperm of the African Blood Lily Haemanthus katherinae observed with phase contrast. The movie begins at prophase and proceeds through metaphase, anaphase, telophase, and cell plate formation. The process is speeded up about 300-fold.

Technical Details

The movie was recorded on 16 mm film in 1956 by A.S. Bajer and J. Mole-Bajer and later transferred to video. See, S. Inoue and R. Oldenbourg, 1998. Mol Biol Cell 9:1603-1607.

Biological Sources

NCBI Organism Classification
Haemanthus katharinae
Cell Type
endosperm
Cellular Component
nuclear chromosome
spindle

Biological Context

Biological Process
mitosis
chromosome organization

Attribution

Names
Shinya Inoue
A.S. Bajer
J. Mole-Bajer
Published
S. Inoue 2008 Collected Works of Shinya Inoue. World Scientific Publishing Company, Singapore, Hackensack, NJ, USA

Imaging

Image Type
recorded image
Imaging Mode
phase contrast microscopy
Parameters Imaged
elastic scattering of photons
Source of Contrast
differences in amount of elastic light scattering
Visualization Methods
visualization of contiguous regions
Processing History
16 mm movie copied to video
Data Qualifiers
processed data

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 720px ——
Y 480px ——
Time -- sec
*CIL – Cell Image Library accession number. Please use this to reference an image.