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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11837*  Cite 
Description

Microtubules in a dominant-negative Rac1(T17N)-expressing PtK1 cell. Microtubules do not undergo retrograde flow, and microtubules rapidly switch between growth and shortening phases with little net change in length over time. The cell was microinjected with X-rhodamine-conjugated tubulin and an EGFP-Rac1(T17N) expression plasmid, and was observed by fluorescence microscopy. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 3 of associated paper (J Cell Biol, 161:845-851, 2003).

Biological Sources
NCBI Organism Classification
Potorous tridactylus
Cell Type
epithelial cell
Cell Line
Pt K1 (NBL-3)
Cellular Component
microtubule
Biological Context
Biological Process
microtubule polymerization
Molecular Function
dominant-negative Rac
Attribution
Names
Torsten Wittmann
Gary M. Bokoch
Clare M. Waterman-Storer
Published
J Cell Biol, 161:845-851, 2003
Pubmed
12796474
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL11837
Archival Resource Key (ARK)
ark:/b7295/w9cil11837
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
X-Rhodamine
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 475px 0.043µm
Y 450px 0.043µm