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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:111*  Cite 

This is a section of methanol-fixed, cryostat-sectioned (10 micrometers thick) adult mouse cervical dorsal root ganglion, labeled with antibodies to peripherin (green) and neurofilament protein NF-M (red) components of the intermediate filament cytoskeleton. Note that small diameter neurons and small-caliber fibers, seen in the lower half of the image, are enriched in peripherin. Large-size neurons and thick-caliber fibers are enriched in NF proteins. Microscope - Zeiss Axioplan I Illumination - Mercury arc lamp Objective - 40X oil/NA 0.9 Intermediate lens between obj and camera - 0.63X Camera - Axiocam MRM CCD Filters: 1. Ex 485/20 BP, Em 515-565 BP 2. Ex 560/40, Em 630/60

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
sensory neuron
Cellular Component
Biological Context
Biological Process
intermediate filament-based process
Molecular Function
structural constituent of cytoskeleton
Linda Parysek
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Alexa Fluor 488
Alexa Fluor 594
primary antibody plus labeled secondary antibody
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
Sample Preparation
coagulating-fixative fixed tissue
Relation To Intact Cell
cryostat-sectioned tissue
Spatial Axis Image Size Pixel Size
Y 1300px 260nm
Y 1030px 260nm