Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11023^* Cite

Description

Transmission electron micrograph showing part of a decondensed mitotic chromosome. The residual protein 'scaffold' (at bottom) is surrounded by a 'halo' of DNA loops 20 to 24 micrometers long. The entire chromosome is seen in the grouped CIL image 11021.

Technical Details

Micrograph from J.R. Paulson and U.K. Laemmli (1977) Cell, 12:817-828, and republished with permission as Figure 131 from Chapter 4 (Nucleus) of 'The Cell' by Don W. Fawcett M.D. This image is the enlarged inset panel from CIL:11021 in this image group.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Line: HeLa
Cellular Component: nuclear chromosome; chromatin

Biological Context

Biological Process: nucleus organization; mitotic metaphase; chromatin organization; chromosome organization

Attribution

Names: J. R. Paulson; U. K. Laemmli; Don W Fawcett
Published: ISBN 0721635849
Published: from: Don W. Fawcett M.D ‘The Cell’ 2nd edition, W.B. Saunders Chapter 4 (Nucleus)
Pubmed: 922894
Link: ASCB BioEDUCATE Program; Cell

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL11023
Archival Resource Key (ARK): ark:/b7295/w9cil11023

Grouping This image is part of a group.

Imaging

Image Type: film
Image Mode: transmission electron microscopy (TEM); detection of electrons
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: uranyl salt
Processing History: Image extracted from .pdf file of 'The Cell'
Data Qualifiers: processed data

Sample Preparation

Methods: unfixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	918px	——
Y	1276px	——