Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:11021^* Cite

Description

Transmission electron micrograph of isolated HeLa cell metaphase chromosome decondensed by removal of histones with dextran sulfate and heparin, centrifuged onto a carbon film and stained with uranyl acetate. The residual chromosome 'scaffold' is surrounded by extended free DNA loops 20 to 24 micrometers long. Area boxed is shown at higher magnification in the grouped CIL image 11023.

Technical Details

Micrograph from J.R. Paulson and U.K. Laemmli (1977) Cell, 12:817-828, and republished with permission as Figure 130 from Chapter 4 (Nucleus) of 'The Cell' by Don W. Fawcett M.D.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Line: HeLa
Cellular Component: nuclear chromosome; chromatin; chromosome scaffold

Biological Context

Biological Process: nucleus organization; mitotic metaphase; chromatin organization; chromosome organization

Attribution

Names: J. R. Paulson; U. K. Laemmli; Don W Fawcett
Published: ISBN 0721635849
Published: from: Don W. Fawcett M.D ‘The Cell’ 2nd edition, W.B. Saunders Chapter 4 (Nucleus)
Pubmed: 922894
Link: ASCB BioEDUCATE Program; Cell

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL11021
Archival Resource Key (ARK): ark:/b7295/w9cil11021

Grouping This image is part of a group.

Imaging

Image Type: film
Image Mode: transmission electron microscopy (TEM); detection of electrons
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: uranyl salt
Processing History: Image extracted from .pdf file of 'The Cell'
Data Qualifiers: processed data

Sample Preparation

Methods: unfixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	900px	——
Y	1272px	——