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Description

Freeze fracture image of the E-face of rat bladder lumenal membrane. The lumenal surface of these epithelial cells is composed of plaques separated by interplaque regions. Within the plaques are hexagonal arrays of subunits (about 12nm, with center-to-center spacing of 15.6nm) on the E-face of the membrane. The inset panel shows these subunits at higher magnification, prepared by deep-etching rather than fracturing after freezing. At higher magnification, each subunit within the plaque appear to be composed of a ring of six particles surrounding a channel. These plaques are distinguished from gap junctions (found on the P-face of membranes) by their location on their position instead on the E-face. These micrographs courtesy of Nicholas Severs and Marian Hicks, Figure 13 from Chapter 1 (The Cell Surface) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.

Technical Details

With the freeze-fracture technique, tissue is rapidly frozen and cracked to shear along zones of weakness. Cleavage of membranes occurs along the hydrophobic interior of the lipid bilayer to reveal views of a "p-face" (the outwardly-facing inner half of the membrane) and an "e-face" (the inwardly-facing outer half of the membrane), and a metallic replica is made of the fractured surface. The intramembranous particles represent integral membrane proteins.

Biological Sources

NCBI Organism Classification
Rattus
(rats)
Cell Type
transitional epithelial cell
Cellular Component
plasma membrane
extracellular region part

Biological Context

Biological Process
plasma membrane organization

Attribution

Names
Nicholas Severs
Marian Hicks
Published
ISBN 0721635849
Link
The Cell, 2nd Ed. by D.W. Fawcett

Grouping

This image is part of a group.
Data Qualifiers
processed data

Sample Preparation

Methods
freeze_fracture/freeze_etch
Relation To Intact Cell
isolated subcellular component

Dimensions

Spatial Axis Image Size Pixel Size
X 902px ——
Y 1272px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.