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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10541*  Cite 
Description

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged TgIMC1 (innermembrane complex scaffold protein) and mCherry-tagged human alpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: mCherry, ex 555-592 em 600-665, YFP, ex 490-510 em 525-595. Captured with CoolSnapHQ/ICX285. This is a maximum intensity projection from z slices 3-6 from deconvolved data stack: CIL# 10539. Maximum intensity projection from z slices 10-13: CIL# 10540. Corresponding raw data stack: CIL# 10538 and raw data stack with DIC: CIL# 10543. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

Biological Sources
NCBI Organism Classification
Toxoplasma gondii RH
Cell Line
Toxoplasma gondii RH stable cell line
Cellular Component
cortical microtubule
spindle pole
centriole
conoid
inner membrane complex
Attribution
Names
John Murray (University of Pennsylvania)
Ke Hu (Indiana University, Bloomington)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL10541
Archival Resource Key (ARK)
ark:/b7295/w9cil10541
Grouping This image is part of a group.
Imaging
Image Type
recorded image
charge coupled device (CCD)
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Yellow fluorescent proteins from Aequorea
mCherryFP
Processing History
3D-deconvolution
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1024px 0.1067µm
Y 1024px 0.1067µm