Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10525^* Cite

Description

Time-lapse of Toxoplasma gondii infection of human foreskin fibroblast cells. T. gondii stably express EGFP-tagged TgIMC1 and mRFP-tagged TgMORN1 (MORN domain containing protein). Formation of daughter cells is visible during time-lapse. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: EGFP, ex 450-490 em 500-540, mRFP, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. 3D stacks of images were collected at 0.6 μ increments in z, every 10 min. Maximum intensity projection of 3D deconvolved stacks at each timepoint were used to generate mpg movie. Corresponds to raw data: CIL# 10525. and 3D deconvolved data: CIL# 10523 and projection of 3D deconvolved data: CIL# 10524. Unpublished image that is similar to images published in PMID: 16518471 and 20140195. See publications for other experimental details.

Biological Sources

NCBI Organism Classification: Toxoplasma gondii RH
Cell Line: Toxoplasma gondii RH stable cell line
Cellular Component: inner membrane complex; centriole; spindle pole; conoid; basal part of cell

Biological Context

Biological Process: regulation of cell shape; cytoskeleton organization; cell division
Human Disease: toxoplasmosis

Attribution

Names: John Murray (University of Pennsylvania); Ke Hu (Indiana University, Bloomington)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10525
Archival Resource Key (ARK): ark:/b7295/w9cil10525

Grouping This image is part of a group.

Imaging

Image Type: recorded image; charge coupled device (CCD)
Image Mode: fluorescence microscopy; time lapse microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP; mRFP1
Processing History: 3D-deconvolution
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	576px	——
Y	416px	——