Licensing

Pd
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Description

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgCentrin2 and mCherry-tagged TgIMC1 (innermembrane complex scaffold protein). Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 100X oil immersion, 1.35 NA. Filters: EGFP, ex 450-490 em 500-540, mCherry, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. 3D stack image is raw data corresponding to deconvolved stack: CIL# 10500. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

Biological Sources

NCBI Organism Classification
Toxoplasma gondii RH
Cell Line
Toxoplasma gondii RH stable cell line
Cellular Component
inner membrane complex
centriole
polar ring of apical complex

Biological Context

Biological Process
regulation of cell shape
cell division
Human Disease
toxoplasmosis

Attribution

Names
John Murray (University of Pennsylvania)
Ke Hu (Indiana University, Bloomington)

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
charge coupled device (CCD)
Imaging Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
mCherryFP
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 128px .1326μm
Y 128px .1326μm
Z 10 .3μm
*CIL – Cell Image Library accession number. Please use this to reference an image.