Licensing

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Description

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgCentrin2 and mCherry-tagged TgIMC1 (innermembrane complex scaffold protein). Images overlaid with DIC. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 100X oil immersion, 1.35 NA. Filters: EGFP, ex 450-490 em 500-540, mCherry, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. Data stack corresponds to CIL# 10495 and deconvolved data stack CIL 10496. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

Biological Sources

NCBI Organism Classification
Toxoplasma gondii RH
Cell Line
Toxoplasma gondii RH stable cell line
Cellular Component
inner membrane complex
centriole
polar ring of apical complex

Biological Context

Biological Process
regulation of cell shape
cell division
Human Disease
toxoplasmosis

Attribution

Names
John Murray (University of Pennsylvania)
Ke Hu (Indiana University, Bloomington)

Grouping

This image is part of a group.
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 256px .0663μm
Y 256px .0663μm
Z 7 .3μm
*CIL – Cell Image Library accession number. Please use this to reference an image.