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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10461*  Cite 
Description

Toxoplasma gondii expressing EGFP tagged Tgbeta1-tubulin and TgAPR1-mCherry (TgAPR1 is a Microtubule Organizing Center protein). Images overlaid with DIC. For GFP, ex 450-490 em 500-540; for mCherry ex 555-592 em 600-665. Images acquired using API DeltaVision on a Olympus ix70 wide-field microscope. Objective: Olympus 60X water immersion, 1.2 NA. Captured with CoolSnapHQ/ICX285. 15 images in z-series; Image stack corresponds to deconvolved data: CIL# 10458 and raw image stack: CIL# 10457. Unpublished image similar to images published in PMID: 16518471. Also see publication for experimental details.

Biological Sources
NCBI Organism Classification
Toxoplasma gondii RH
Cell Type
Toxoplasma gondii RH tachyzoite
Cell Line
Toxoplasma gondii RH double stable clone expressing TgAPR1-mCherry and EGFP-beta1-tubulin
Cellular Component
cortical microtubule cytoskeleton
microtubule organizing center
Biological Context
Biological Process
regulation of cell shape
Molecular Function
structural constituent of cytoskeleton
Human Disease
toxoplasmosis
Attribution
Names
John Murray
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL10461
Archival Resource Key (ARK)
ark:/b7295/w9cil10461
Grouping This image is part of a group.
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 106.7nm
Y 512px 106.7nm
Z 15px 300nm


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