Licensing

Pd
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Description

Toxoplasma gondii expressing EGFP tagged Tgbeta1-tubulin and TgAPR1-mCherry (TgAPR1 is a Microtubule Organizing Center protein). Images overlaid with DIC. For GFP, ex 450-490 em 500-540; for mCherry ex 555-592 em 600-665. Images acquired using API DeltaVision on a Olympus ix70 wide-field microscope. Objective: Olympus 60X water immersion, 1.2 NA. Captured with CoolSnapHQ/ICX285. 15 images in z-series; Image stack corresponds to deconvolved data: CIL# 10458 and raw image stack: CIL# 10457. Unpublished image similar to images published in PMID: 16518471. Also see publication for experimental details.

Biological Sources

NCBI Organism Classification
Toxoplasma gondii RH
Cell Type
Toxoplasma gondii RH tachyzoite
Cell Line
Toxoplasma gondii RH double stable clone expressing TgAPR1-mCherry and EGFP-beta1-tubulin
Cellular Component
cortical microtubule cytoskeleton
microtubule organizing center

Biological Context

Biological Process
regulation of cell shape
Molecular Function
structural constituent of cytoskeleton
Human Disease
toxoplasmosis

Attribution

Name
John Murray

Grouping

This image is part of a group.
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 512px 106.7nm
Y 512px 106.7nm
Z 15 300nm
*CIL – Cell Image Library accession number. Please use this to reference an image.