Licensing

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Description

Toxoplasma gondii double stable clone expressing TgAPR1-mCherryFP (an MTOC protein) and EGFP-beta1-tubulin. 3D stack image of a live unfixed specimen acquired using API Delta Vision on Olympus ix70 wide-field microscope with Cool SNAP HQ/ICX285 CCD camera. Filter set: EGFP excitation 450-490 and emission 500-540; mCherry excitation 555-592 and emission: 600-665. Dichromatic mirror: Chroma 89021. Objective: Olympus 60x water immersion, 1.2 NA. This raw data stack with DIC corresponds to raw data stack: CIL# 10454 and deconvolved stack: CIL# 10455. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

Biological Sources

NCBI Organism Classification
Toxoplasma gondii RH
Cell Type
Toxoplasma gondii RH
Cell Line
Toxoplasma gondii RH double stable clone expressing TgAPR1-mCherryFP and EGFP-beta1-tubulin
Cellular Component
cortical microtubule, transverse to long axis
microtubule organizing center

Biological Context

Biological Process
regulation of cell shape
Molecular Function
structural constituent of cytoskeleton
Human Disease
toxoplasmosis

Attribution

Name
John Murray

Grouping

This image is part of a group.
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 384px 106.7nm
Y 384px 106.7nm
Z 15 300nm
*CIL – Cell Image Library accession number. Please use this to reference an image.