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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10460*  Cite 
Description

Toxoplasma gondii double stable clone expressing TgAPR1-mCherryFP (an MTOC protein) and EGFP-beta1-tubulin. 3D stack image of a live unfixed specimen acquired using API Delta Vision on Olympus ix70 wide-field microscope with Cool SNAP HQ/ICX285 CCD camera. Filter set: EGFP excitation 450-490 and emission 500-540; mCherry excitation 555-592 and emission: 600-665. Dichromatic mirror: Chroma 89021. Objective: Olympus 60x water immersion, 1.2 NA. This raw data stack with DIC corresponds to raw data stack: CIL# 10454 and deconvolved stack: CIL# 10455. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

Biological Sources
NCBI Organism Classification
Toxoplasma gondii RH
Cell Type
Toxoplasma gondii RH
Cell Line
Toxoplasma gondii RH double stable clone expressing TgAPR1-mCherryFP and EGFP-beta1-tubulin
Cellular Component
cortical microtubule, transverse to long axis
microtubule organizing center
Biological Context
Biological Process
regulation of cell shape
Molecular Function
structural constituent of cytoskeleton
Human Disease
toxoplasmosis
Attribution
Names
John Murray
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL10460
Archival Resource Key (ARK)
ark:/b7295/w9cil10460
Grouping This image is part of a group.
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 384px 106.7nm
Y 384px 106.7nm
Z 15px 300nm