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Mouse tracheal epithelia cells (MTEC) were grown at air-liquid interface to induce differentiation, centriole amplification and ciliogenesis. Cells were fixed with ice-cold methanol for 10 min. Samples were stained with antibodies against acetylated tubulin to mark cilia (red), the centriolar protein Cep120 to mark basal bodies (green), and anti-ZO-1 to mark the cell-cell junctions (light blue). Secondary antibodies were Alexa 594 for acetylated tubulin, Alexa 488 for Cep120, and Cy5 for Zo-1. Nuclei were stained using DAPI (blue). Images were captured using Openlab 4.0.4 software controlling an Axiovert 200M microscope (Carl Zeiss, Inc.) with a 100X 1.4 NA objective.

Biological Sources

NCBI Organism Classification
Mus musculus
(house mouse)
Cell Type
tracheal epithelial cell
Cellular Component
cell-cell junction
microtubule basal body
acetylated tubulin

Biological Context

Biological Process
lung ciliated cell differentiation


Moe Mahjoub
Tim Stearns


This image is part of a group.

Sample Preparation

methanol fixed tissue
Relation To Intact Cell
detergent permeabilized


Spatial Axis Image Size Pixel Size
X 1171px 0.094μm
Y 1017px 0.094μm
Channel Wavelength
1 350, 488, 594, 633 nm
*CIL – Cell Image Library accession number. Please use this to reference an image.