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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10061*  Cite 

Mouse tracheal epithelia cells (MTEC) were grown at air-liquid interface to induce differentiation, centriole amplification and ciliogenesis. Cells were fixed with ice-cold methanol for 10 min. Samples were stained with antibodies against acetylated tubulin to mark cilia (red), the centriolar protein Cep120 to mark basal bodies (green), and anti-ZO-1 to mark the cell-cell junctions (light blue). Secondary antibodies were Alexa 594 for acetylated tubulin, Alexa 488 for Cep120, and Cy5 for Zo-1. Nuclei were stained using DAPI (blue). Images were captured using Openlab 4.0.4 software controlling an Axiovert 200M microscope (Carl Zeiss, Inc.) with a 100X 1.4 NA objective.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
tracheal epithelial cell
Cellular Component
cell-cell junction
microtubule basal body
acetylated tubulin
Biological Context
Biological Process
lung ciliated cell differentiation
Moe Mahjoub
Tim Stearns
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Sample Preparation
methanol fixed tissue
Relation To Intact Cell
detergent permeabilized
Spatial Axis Image Size Pixel Size
X 1171px 0.094µm
Y 1017px 0.094µm
Channel Wavelength
1 350, 488, 594, 633nm